SUBTYPE DETECTION OF AVIAN INFLUENZA NEURAMINIDASE WITH NEURAMINIDASE INHIBITION TEST

Publish Year: 1397
نوع سند: مقاله کنفرانسی
زبان: English
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MEDISM19_671

تاریخ نمایه سازی: 13 مهر 1397

Abstract:

Background and Aim:Neuraminidase(NA) is a glycoprotein expressed on the surface of influenza viruses. There are 9 known subtype of NA based on serological cross reactivity. Characterization of influenza virus isolates is frequently done by both classical and molecular methods. Classical methods such as Hemagglutination Inhibition (HI) assay and neuraminidase inhibition assay are still the reference standards required by OIE. The neuraminidase-inhibition (NI) test is a laboratory and serological procedure for identification and classification of AIVs. Our primary objectives were setting up the NI test for detection of NA subtype in viral or antiserum samples and validate the results with RT-PCR.Methods:Based on OIE protocol the test was experienced for N1,N2,N6 antigens or their antiserum. we used viral isolates of H9N2 and H9N2 field antiserum and standard H5N1 antigen with its standard and field antiserum, standard H5N6 with standard antiserum. Samples were also investigated for NA subtype by RT-PCR reaction. Fetuin (substrate) was added to virus and unknown antiserum or the revers, after incubation sodium periodate then sodium arsenite , and lastly 2-thiobarbituric acid was added. If no or white color was produced the unknown virus or antiserum was considered to be as same as standard samples. But appearance of pink color mean the sample wasn’t the same subtype with standards.

Authors

Najmeh Motamed

Avian Diseases Research department,Razi Vaccine and Serum Research Institute, Agricultural Research,Education and Extension Organization, Karaj,Iran

Abdelhamid Shoushtari

Avian Diseases Research department,Razi Vaccine and Serum Research Institute, Agricultural Research,Education and Extension Organization, Karaj,Iran

Mohammad Hosein Fallah Mehrabadi

Avian Diseases Research department,Razi Vaccine and Serum Research Institute, Agricultural Research,Education and Extension Organization, Karaj,Iran