FIRST REPORT ON ISOLATION OF CHLAMYDIA FELIS FROM IRAN

Publish Year: 1397
نوع سند: مقاله کنفرانسی
زبان: English
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MEDISM19_689

تاریخ نمایه سازی: 13 مهر 1397

Abstract:

Background and Aim:Chlamydiae are gram-negative obligate intracellular bacteria that affects both human and animal health. According to the latest classifications, the order Chlamydiales consists of one family, the Chlamydiaceae, containing one genus, the Chlamydia, which consists of 12 different species that are responsible for a wide range of diseases. Feline chlamydiosis is one of the most important upper respiratory tract diseases of cats. In this paper the successful isolation of C. felis from a cats affected with conjunctivitis is reported.Methods:Several conjunctival swabs were taken from both eyes of a 2-month-old female kitten with a history of severe bilateral conjunctivitis. The samples were used for direct microscopic examination, microbial cultures, and molecular diagnosis of C. felis infection and the case was treated ophthalmic tetracycline ointment twice daily for 1 week.Results:Microbiological cultures of swabs did not yielded the growth of extra-cellular pathogenic bacteria or fungi. Molecular diagnosis of C. felis infection by conventional PCR with C. felis specific primers revealed the presence of targeted gene. The Gimsa and imunoperoxidase staining of smears also showed the specific intra-cytoplasmic chlamydial inclusions. Chlamydia felis was isolated from samples by inoculation to cycloheximide-treated L 929 cells. The symptoms and clinical signs of affected kitten disappeared after treatment.Conclusion:To the best of our knowledge, this is the first reports on isolation of C. felis from Iran. The use of an effective C. felis vaccine for preventing infections in cat populations seem to be necessary and should be recommended.

Authors

Masoud Ghorbanpoor

Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz

Bahman Mosalanejad

Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz

Sara Barati

Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz

Babak Barimani

Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz