SEROLOGICAL SURVEY OF AVIAN INFLUENZA (H9N2) IN INDUSTRIAL POULTRY IN GOLESTAN PROVINCE DURING 2017-2018

Background and Aim:Influenza is an acute respiratory disease caused by infection with influenza viruses. Avian influenza (AI) viruses are members of the family Orthomyxoviridae. AI viruses can be classified into two categories: low pathogenicity (LPAI) and highly pathogenic (HPAI) forms, based on the severity of the illness caused in birds. The aim of this study was to monitor mean antibody titer of influenza virus (H9N2) in commercial farms in Golestan province using HI assays.Methods:in this study we screened the serum antibody level in 17 epidemiologic units and from each unit Collected 15 to 20 samples. Blood samples were collected from the biliary vein by a 2-cc syringe of blood and the syringe was kept at room temperature for 2 hours and transferred to the refrigerator at 4° C. The Hemagglutination Inhibition (HI) test was then performed to determine the titre of antibody.Results:from the 17 epidemiologic units 9 unit the titre of HI low and need to vaccination for prevention of H9N2 virus. HI tests showed that the lowest and the highest mean antibody titer of AI was 3.63 with C.V 30% and 6.91 with C.V 8.3 %, respectively.Conclusion:The findings of this study not only reveal low prevalence of AIV (H9N2) antibodies in Poultry farms in Golestan province, but also emphasize for increase the serum antibody level with good vaccination program for improve poultry immune systems.

Keywords:

H9N2 , HI , Golestan , poultry