Zahra Moghimi - Student of pharmacy,Ahvaz Jundishapur University of Medical sciences,Ahvaz,Iran
Golshan Sheikhi - Pharmacist,Ahvaz Jundishapur University of Medical sciences,Ahvaz,Iran
Abdolghain Ameri - Microbiology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Sepide Sayadi - Pharmacist,Ahvaz Jundishapur University of Medical sciences,Ahvaz,Iran

Background and Aim:Salmonella is one of the most common bacterium causing gastrointestinal infections. Nowadays the resistance of Salmonella against current antibiotics is increasing day by day. Nowadays tendency toward using the natural, biologic and probiotic products as substitutions of antibiotics have been increased due to prevalent side effects of using antibiotics and resistance against them. Probiotic bacteria are living microorganisms which fight against pathogens using different mechanisms.Methods:The lactobacillus .Casei was cultured in MRS media under microaerophile conditions and its extract was gathered by centrifuge. At first its acidity and salmonellosis effect in comparison with neutral and alkaline states was examined by method of cup plate. The minimum restrainability concentration of probiotic extract was examined using method of serial micro dilution in concentration range of 0.0625 – 1 mg/ml. A comparison had been made using anti biogram test between anti pathogen effect of probiotic extract and common antibiotics in treatment.Results:The results of the minimum restrainability concentration showed that the stable extract had more antibacterial effect in comparison with supernatant (P˂0/01). Using anti biogram test and comparing anti pathogen effect of probiotic extract and common antibiotics revealed that the effect of stable extract is more than supernatant and some antibiotics.Conclusion:This research shows that useful effect of probiotic against salmonella is related to its metabolites and the stability and effect of stable extract is more than supernatant.

Probiotic – lactobacillus – Salmonella