MOLECULAR IDENTIFICATION AND ISOLATION OF DAIRY PRODUCTS’ PROBIOTIC LACTOBACILLI FROM FARS, IRAN

Background and Aim:Probiotics are live microorganisms that can be found in the natural flora of the human intestine. Lactobacilli as a type of probiotics are useful for human health. Several studies have reported the effects of lactobacilli and their products including toxins, LTA, LPS and glycoproteins on the metabolism, inflammation, cellular and immune response, skin restoration, improvement of cancer and HIV. The purpose of this study was to isolate and identify the probiotic Lactobacilli extracted from the dairy products of Fars province, Iran.Methods:This bacterium is naturally present in dairy products, therefore, native dairy products of Fras province, Iran were collected. The bacteria of these products were cultured in MRS broth and MRS agar media. After the growth, in order to identify and purify the bacteria, chemical methods including Gram staining and TSI and other tests to evaluate the Catalase and oxidase activity were performed. Thereafter, several strains of lactobacillus were identified using the molecular method PCR analysis (16S rDNA).Results:: Different strains of lactobacilli were identified, purified and replicated from the native dairy products of Fars province.Conclusion:Regarding the fact that probiotic-extracted lactobacilli and their products can be used for biomedical and healthcare applications, it is important to identify the dairy products bacterium strains of any specific area for further researches.

Keywords:

probiotics , lactobacillus , dairy products