DISCRIMINATION OF LACTOBACILLUS SPECIES EXTRACTED FROM TRADITIONAL DAIRY PRODUCTS USING RAPD-PCR MOLECULAR MARKER

Publish Year: 1397
نوع سند: مقاله کنفرانسی
زبان: English
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MEDISM19_728

تاریخ نمایه سازی: 13 مهر 1397

Abstract:

Background and Aim:Probiotics are non-pathogenic useful microorganisms with positive effects on the hos health.the aim of the present study was to discriminate Lactobacillus species extracted from traditional dairy productsMethods:This study was conducted on twenty-six specimens were collected from traditional dairy products in Bukan. Lactobacillus species were separated and purified by employing biochemical tests. Then, the intra/inter-species diversity was investigated using the RAPD-PCR technique.Results:PIC value varied between 15.9 and 34.4 % with the maximum value of 34.4 percent associated with the 1254 primer. The mean of MI for the 6 primers was 4.52, in which the maximum and minimum values belonged to the 1254 and OPA-02 primers, respectively. UPGMA clustering method based on Jaccard similarity coefficient categorized the isolates into four main clusters. Principle Coordinates analysis (PCoA) demonstrated that the first and second components explained 30.59% and 22.48% of variances, i.e. 53.07% of variances in total. the results of RAPD marker indicated that the intra-species diversity was greater inter-species diversity. The intra-group variance explained 94 percent of the all variance, while inter-group variance explained 6 percent of the all variance. Moreover, the results of AMOVA indicated that the highest level of discrimination was occurred at the 16 groups cut-off point with a similarity coefficient of 0.56.Conclusion:It can be concluded from the present study that are an enriched source of probiotic bacteria which can ensure the health of general population and enhance their immune systems. Moreover, RAPD-PCR is an appropriate method for detection and classification of lactobassiluses.

Authors

Seyed Mostafa Hosseini

Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, IR Iran

Mohammad Reza Arabestani

Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, IR Iran