Seyed Mostafa Hosseini - Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, IR Iran
Mohammad Reza Arabestani - Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, IR Iran

Background and Aim:Probiotics are non-pathogenic useful microorganisms with positive effects on the hos health.the aim of the present study was to discriminate Lactobacillus species extracted from traditional dairy productsMethods:This study was conducted on twenty-six specimens were collected from traditional dairy products in Bukan. Lactobacillus species were separated and purified by employing biochemical tests. Then, the intra/inter-species diversity was investigated using the RAPD-PCR technique.Results:PIC value varied between 15.9 and 34.4 % with the maximum value of 34.4 percent associated with the 1254 primer. The mean of MI for the 6 primers was 4.52, in which the maximum and minimum values belonged to the 1254 and OPA-02 primers, respectively. UPGMA clustering method based on Jaccard similarity coefficient categorized the isolates into four main clusters. Principle Coordinates analysis (PCoA) demonstrated that the first and second components explained 30.59% and 22.48% of variances, i.e. 53.07% of variances in total. the results of RAPD marker indicated that the intra-species diversity was greater inter-species diversity. The intra-group variance explained 94 percent of the all variance, while inter-group variance explained 6 percent of the all variance. Moreover, the results of AMOVA indicated that the highest level of discrimination was occurred at the 16 groups cut-off point with a similarity coefficient of 0.56.Conclusion:It can be concluded from the present study that are an enriched source of probiotic bacteria which can ensure the health of general population and enhance their immune systems. Moreover, RAPD-PCR is an appropriate method for detection and classification of lactobassiluses.

Probiotic, Lactobacillus, RAPD-PCR