ANTIMICROBIAL ACTIVITY AND ANTIBIOTIC SUSCEPTIBILITY OF BIFIDOBACTERIUM SPP ISOLATE FROM HUMAN MILK AGAINST HOSPITAL , ENTEROPATHOGENIC AND FOOD-BORNE PATHOGENS

Background and Aim:Human breast milk consists of high amounts of necessary nutrients and various bioactive for infants . Among these bioactive agents, probiotic bacteria were isolated from human milk.The research comprises isolation and identification of Bifidobacterium strains from Human Milk.Methods:samples cultured in MRS broth, Bifido agar under anaerobic conditions.Identification of Bifidobacterium isolates was performed by biochemical and PCR technique using species specific Primers . An agar well diffusion assay , disc diffusion method and Elisa method was used for detection of antimicrobial activity,Antibiotic susceptibility and Aflatoxin B1 detoxification potential respectively.Statistical analyses were performed with SPSS software.One-way ANOVA with post-hoc Tukey HSD was used for statistical analysis. Results were regarded as statistically significant at p< 0.05Results:The examined strains were identified as B. Longum , B. Breve .All Isolate showed good probiotic potential.The majority of the strains exhibited antagonistic activity towards Bacillus Cereus , salmonella , Campylobacter , E.coli , shigella , staphylococcus areus respectively . all strains tested were susceptible to penicillin G, amoxicillin, piperacillin, ticarcillin, imipenem, chloramphenicol, rifampicin and vancomycin . all strains tested were resistant to gentamicin, sulfamethoxazole and polymyxin B . Most isolates were resistant to fusidic acid .Conclusion:This study showed that Bifidobacterium strains with good probiotic potential could be isolated from Human milk and suggest Bifidobacterium strains with probiotic potential may be useful for prevention or treatment of diarrhea and Supportive therapy in Aflatoxicosis but further in vitro and in vivo studies on these strains are still required.

Keywords:

Bifidobacterium , human milk , Probiotics , Antimicrobial Activity