a mohammadi - Associate Professor- Head of Human Viral Vaccine Production Department, Razi Vaccine and Serum Research Institute (RVSRI) Hessark Karadj, Agricultural Research, Education a nd Extension Organization (AREEO), Tehran, Iran.
a fouroughi - Associate Professor- Head of Human Viral Vaccine Production Department, Razi Vaccine and Serum Research Institute (RVSRI) Hessark Karadj, Agricultural Research, Education a nd Extension Organization (AREEO), Tehran, Iran.
r ghorbani - Scientific Member of Human Viral Vaccine Production Department, Razi Vaccine and Serum Research Institute (RVSRI) Hessark Karadj, Agricultural Research, Education andExtension Organization (AREEO), Tehran, Iran.
b alirezaei - Scientific Member of Human Viral Vaccine Production Department, Razi Vaccine and Serum Research Institute (RVSRI) Hessark Karadj, Agricultural Research, Education andExtension Organization (AREEO), Tehran, Iran.

Background: Cell Line Resource Service centres (CLRSc) have made myriad contributions to society and science. In this article, the isolation and establishment of a new cell line are presented. This cell substrate made possible the isolation and detection of viral strains, helped in the development of vaccines for animals and humans and maintained and provided reference cell lines to ensure the quality and reproducibility of biological. However, many cell banks have grown to help large researches for human being. Moreover, several international organisations have described CLRSs as a factor in economic and scientific development, leading to increased professional use as a tool for diagnostics or the development of biological products.Materials and Methods: Different sample of Human Lung tissue, were surveyed for this research. After deriving the cell line, the first seven endpoints (cell viability, cloning, Isoenzyme assay, species identification and evaluation of cross contamination)are discussed in detail in this article for new cell line. Viral sensitivity produced by inoculating the virus and/or its adaptation was confirmed by heamadsorption,the cytopathogenic appearance of the virus or immunoflurocent testing.Results: The analysis revealed that the isolated human cell line were not contaminated with adventitious agents and were sensitive to a different panel of viruses.Conclusion: In this study, we prepared cell line from different tissues and characterized, authenticated, inoculated and evaluate its sensitivity to various viruses. The isolated cell lines were passaged several times to produce enough stocks in liquid nitrogen for cryopreservation

cell lines; Human/ origin; RHDC; Authentication; sensitivity