The Effects of combination therapy of Diazepam and Glibenclamide on Catalase in Heart Tissue among Streptozotocin-Induced Diabetic Rats

Background and Objective:Diabetes mellitus is a metabolic disorder with chronic hyperglycemia and defects in carbohydrates, lipids and proteins metabolism. Glibenclamide is one of the material that is widely used in diabetes. Other material that is widely used in various countries is diazepam because of its role on reducing stress but the effects of diazepam on the role of glibenclamide therapy is unknown. This study was conducted to survey the combination therapy effect of diazepam and glibenclamide on oxidative stress of heart tissue among diabetic rats. Materials and Methods:In this experimental investigation, 32 male rats, weighing 200 - 250 g, were divided into four groups: 1: diabetic control without treatment, 2: diabetic receiving treatment with glibenclamide, 3: diabetic receiving treatment with diazeapm, and 4: diabetic receiving treatment with glibenclamide and diazepam. Diabetes was induced by intraperitoneal injection of streptozotocin (60 mg/kg). On the third day after the injection of streptozotocin, to ensure the mice are diabetic, blood glucose levels were measured from the tail and glucose more than 250 mg /dl showed that the mice are diabetic. Then treatment began. Treated group with glibenclamide get daily 0.3cc, treated group with diazepam get daily 0.2cc and the group treated with glibenclamide and diazepam get daily respectively, 0.15cc and 0.1cc by intraperitoneal injection. . In the second week after drug administration, rats were anesthetized and by opening the peritoneal cavity their heart removed and after homogenizing and centrifuging, by using supernatant, Catalase was measured by Beer & Sizer method. Quantitative data were analyzed using one-way ANOVA. Results: Blood samples from the tail, representing all groups are diabetic. By measuring the heart tissue catalase activity, it was found that diabetic rats treated withglibenclamide resulted a significant increase in activity of enzyme compared to the diabetic group (p<0.01). Diabetic rats treated with diazepam also has a significant increase in enzyme activity level, but lower compared to the diabetic group (p<0.05). And diabetic rats treated with the drug combination of diazepam and glibenclamide also significantly increased the enzyme activity compared to the diabetic group (p<0.01) However, this increase wasn’t more than the diabetic group treated with glibenclamide.Conclusion: In short, it was found that using glibenclamide has a beneficial effect, also using diazepam or the combination has a beneficial effect in catalase level, however this effect isn’t more than glibenclamide effect.

Keywords:

Diabetes Mellitus , heart , catalase , diazepam , glibenclamide , Rat