Effect of Selenium Biogenic nanoparticles on ERG11 and CDR1 Candida albicans, resistant to fluconazole by Real Time PCR

Background and objective: C. albicans spp. remains as opportunistic yeast that most isolated from fungal infections. The resistant to azole has been considerably rising in the last decades. The toxicity of antimicrobial drugs, resistance to antifungals and drug interactions, highlights the importance of considering new drug combinations. The studies also show wide biological effects of nanoparticles. The aim of this study was to assess the in- vitro effects of nano selenium as an antifungal drug on azole-resistant and susceptible Candida ablicans. Materials and methods: Selenium nanoparticles synthesized with growth of Bacillus sp. Msh-1 was in nutrient medium. The specification of selenium nanoparticles was performed with Electron Microscopy (TEM). The antibiotic susceptibility test of selenium nanoparticles for azole resistance C. albicans (ATCC76615) and wild type C. albicans (ATCC 10231) isolates was performed according to the CLSI M27-A3 standard protocol. The RNA of isolates was prepared with to the manufacturer’s instruction. Synthesis of cDNA was conducted with the 1621K kit (Life Sciense kit) and according to manufacturer’s instruction. The RT-PCR reaction was set up for CDR1 and ERG11 genes and the housekeeping gene β-actin was amplified as Reference Gene. Results: The azole resistance C. albicans and wild type C. albicans isolates were inhibited to with 100μg/ml and 70μg/ml of nano selenium concentration, respectivly. The expression of CDR1 and ERG11 genes was significantly increased when usingnano selenium at the concentration concentration of 100μg/ml and 70μg/ml. Conclusion: The present study exhibited that selenium nanoparticles have an appropriate antifungal activity against fluconazole resistant and susceptible Candida albicans strains. In the future, the production of nanoparticles with biological methods due to their low toxicity and low cost is a good alternative to producing other methods of producing nanoparticles.

Keywords:

Candida ablicans , selenium nanoparticles , azole-resistance