The effect of chlorogenic acid on stress oxidative in rats with cognitive deficit induced by intracerebroventricular streptozotocin

Background and Objectives: Sporadic Alzheimer’s type dementia is more abundant than other dementia diseases which causes memory and multiple cognitive damagesand neurological disorders. Many pathological conditions such as increased oxidative stress, amyloid β (Aβ) plaque formation, neuroinflamation results in AD. Oxidativestress plays a major role in the pathogenesis of Alzheimer s disease. Intracerebroventricular STZ injection decrease cerebral glucose uptake and produce Alzheimer’s disease. Chlorogenic acid (CGA) is a polyphenol that is found in diverse agricultural products such as coffee, beans, potatoes, and apples which reported to possess potent antioxidant and neuroprotective properties. The present study demonstrates the effect of CGA on oxidative stress activity in rats with cognitive deficit induced by intracerebroventricular STZ. Materials and methods: In this experimental study, 32 male rats (250-290g) were divided to four different groups having 8 rats in each group as: group A (control),group B (Control+ CGA), group C (STZ), group D (STZ+CGA). STZ was injected (bilaterally, 3 mg/kg body weight, on days 1 and 3). CGA was administered through intraperitoneal rout at a dose of 50 mg/kg on 7th day after surgery and continued up to 14 days. Oxidative stress markers were determined in cortical and hippocampal brain regions of rats.We measured activities of the enzymes Catalase (CAT), Glutathione reductase (GSH), Malondialdehyd (MDA). Result and conclusion: GSH and CAT activities in STZ group compared to control group had been decreased. CGA+STZ group showed significant increase in enzymatic activity. MDA levels in STZ group compared to control group had been increased. Treatment with CGA decreased MDA level in STZ+CGA group. Therefore, CGA could have been anti-oxidant and neuroprotective activity.

Keywords:

Alzheimer , streptozotocin , Chlorogenic acid