Mustafa Abd Manshood - College of Agriculture, University of Muthanna
Mohammad M.F. Al-Halbosiy - Biotechnology Research Centre, University of Al-Nahrain
Mohamd M. Radhwan - College of Agriculture, University of Muthanna

This study investigated the role of ethanolic extracts of four plants are Artemisia sieberi, Sonchus oleraceus, Savignya parviflora,and Reomeria hybrida as a cytotoxic agent against RAW264.7 cell line using different concentrations of this extracts 6.25, 12.5 , 25 and 50 μg / ml for exposure time 24 hour. The results revealed a clear cytotxoic activity of this extract on growth of RAW264.7 cell line, and the effect was concentration-dependent. The signification inhibition (P<0.05) was obtained at a concentration of50 and 25 μg / ml as compared to control for all extracts. In addition, the growth of RAW264.7 cell line was more sensitive to Sonchus oleraceus and Savignya parviflora in comparison with Artemisia sieberi and Reomeria hybrida . In conclusion, the extracts showed inhibitory effect on RAW264.7 cell line and it is promising to use as anticancer drug.

A. sieberi, S. oleraceus, S. parviflora,and R. Hybrida cytotoxicity, RAW264.7 cell