Investigation of the Antibacterial Role of Prunus Domestica L. Wall Nuts Polyphenolic Extracts

The present study investigates the effect of polyphenolic extracts of Prunus domestica red (PDR) and Prunus domestica yellow (PDY) wall nuts on acute toxicity in female rats and anti-bacterial in vitro. The working of this thesis involves the following terms: Analytical study: involved extraction, isolation and identification of polyphenolic content of PDR and PDY extracts. The UV-Vis Spectra and high performance liquid chromatography of extracted polyphenols are proved the presence of (tannic acid, gallic acid, caffeic acid, vanillic acid, ferulic acid, chlorogenic acid and amygdalin) in both extracts.Acute toxicity: in vivo study included of both extracts was performed on four groups of rats (6 rats in each group). After treatment of different concentration of PDR and PDY polyphenolic extracts (25,50 and 100 mg/kg B.W) and after 72 hr of treatment, no mortality in all rats of experiments can be observed. This indicated that both extracts are orally non toxic.Antibacterial study: four types of bacteria, two Gram positive (Staphylococcus aureus, Bacillus subtilus) and two Gram negative (Escherichia coli, Pseudomonas aeruginosa) have been used to investigate the antibacterial activity of both extracts. Both extracts are actively worked against both types of bacteria. Furthermore, the highest activity of extracted polyphenolic for PDR against Pseudomonas aeruginosa (30 mm) is used, whilst the same extraction had equally inhabited zone against Escherichia coli, Staphylococcus aureus and Bacillus subtilus (20 mm). Finally, for extracted polyphenolic of PDY founded that highest activity against Staphylococcus aureus (13 mm) and lowest activity against Bacillus subtilus.

Keywords:

Prunus domestica L. , polyphenols , acute toxicity , anti-bacterial