Enzyme Polygalacturonase production by fungal strains using agroindustrial bioproduct in solid state fermentation
Publish place: National Conference on Chemical Engineering
Publish Year: 1388
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
NCCEESLAMSHAR01_127
تاریخ نمایه سازی: 9 آبان 1388
Abstract:
Polygalacturonase production by isolated fungal strains was carried out in solid state fermentation. Aspergillus niger (A.niger) and Penicillium sp EGC5 produced polygalacturonase (PG) on mixture of apple bagasse and wheat bran. The mixture of apple bagasse and wheat bran acted as a good nutrient source and substrate for the cultivation of the microorganisms and polygalacturonase produced in solid state fermentation. In this respect, it was possible to obtain polygalacturonase activity at an acceptable yield, in comparison with a typical defined medium described in the literature for polygalacturonase production. Higher titres of polygalacturonase were observed when medium was supplemented with carbon ( pectin) and nitrogen ((NH4)2SO4 and peptone) sources. The maximum production of polygalacturonase was reached after 8 days cultivation. The temperature was 30 ?C and the relative humidity of the fermentation medium was 70%.
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Authors
Heidar Abbasi
Chemical Engineering Department, Faculty of Engineering, Shahid Bahonar University of Kerman, Jomhuri Eslami Blvd., Kerman, Iran
Arash Dehghani
Chemical Engineering Department, Shiraz University
Hojat Shafighzadeh
Chemical Engineering Department, Malek Ashtar University
Shahryar Amirahmadi
Chemical Engineering Department, Persian Gulf University of Bushehr
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