Malihe Looti bashiyan - Institute for Cognitive Science Studies (ICSS), Tehran, Iran
Mohammad Nasehi - Cognitive and Neuroscience Research Center (CNRC), Amir-Almomenin Hospital, Tehran. Medical Sciences Branch, Islamic Azad University, Tehran, Iran.
Solmaz Khalifeh - Cognitive and Neuroscience Research Center (CNRC), Amir-Almomenin Hospital, Tehran. Medical Sciences Branch, Islamic Azad University, Tehran, Iran
Mohammad-Reza Zarrindast - Institute for Cognitive Science Studies (ICSS), Tehran, Iran

In recent years, crocin has been used for its pharmacological functions such as memory and learning enhancement. Based on the available evidence, the aim of the present study was to assess the effect of crocin on impairment of memory induced by total sleep-deprivation (TSD) in male Wistar rats (weighted 200-230 gr). To this end, the water box apparatus was used to induce sleep deprivation; meanwhile, Y- maze task was used as an index of learning and memory. The rats were divided into 12 groups, 8 rats in each group: four control groups, four sham groups, and four TSD groups. Each group was received saline and crocin at doses of 1, 5, and 15 mg/kg twice a day. The findings revealed that TSD for 24 hrs impaired memory function. In addition, the intra-peritoneal injection of crocin at all doses (1, 5, 15 mg/kg) did not alter and restored the percentage of time spent in the novel arm of Y-maze in sham of TSD and TSD groups, respectively. Based on the results, the findings showed that crocin could restore the amnesia induced by TSD 24 hrs.

Total sleep deprivation, Crocin, Memory, Y-Maze, Rat