Application of Black Carrot Anthocyanin for Wound Healing

Wounds heal through 4 overlapping phases. Any interruption to these pathways leads to non-healing wound. One of the inhibitors of the wound healing process is infection. The need for detection of infection stems from the complexity of not only the dynamic wound infection and healing biochemistry but also the distinguishing normal wound drainage from initial inflammatory response and from infectious process. It seems a quick diagnosis of infection can be helpful in improving wound healing, eliminating unnecessary use of antibiotics, preventing the bacterial resistance and reducing in costs. The aim of this article is to investigate the ability of pH sensitive gelatinous hydrogel integrated with black carrot anthocyanin for early detection of infection. The present article also studies the effect of anthocyanin on different cellular aspects. As an initial step for developing hydrogel for detection of infection, the in vitro biocompatibility of hydrogel was evaluated using L929 mouse fibroblasts. The results provide evidences of good adhesion, spreading, viability and morphology. 6 h after cells seeding on the samples, they were well attached to the surface of the hydrogel and 48 h after seeding they were spread over the surface and connected with each other. The results of the FTIR/ATR analysis showed anthocyanin could inhibit glutaraldehyde free radicals residual. Anthocyanin also could change its color after addition of bacterial supernatant. Thus, hydrogel containing black carrot anthocyanin may be a therapeutic and diagnostic strategy to promote wound healing and early detection of infection.

Keywords:

Wound Healing , Infection , Anthocyanin , pH