Optimization of Cell Proliferation and Repair with the Help of Microcarrier-based Cell Culture Technology

Recently, Microcarrier-based cell culture method has been developed to providing a high surface area for sufficient cell proliferation of the specified tissues. Adhesion and harvesting of cells to and from microcarriers are two critical stages influencing final cell productivity and quality. After a comprehensive study on these crucial factors, all of cellular mechanisms which involved in the attachment and detachment process such as seeding conditions, chemical and mechanical harvesting methods and microcarriers’ surfaces treatments were reviewed and discussed. The results of studies demonstrated that, development of cost-effective biodegradable microcarriers such as gelatin coated Ca- alginate hydrogels can be applied as biocompatible microcarries in tissue engineering applications and employment of other external-stimuli-responsive materials in microcarrier technology are suggested for future works. However, to achieve optimum results, a better understanding of thermodynamic forces involved in the attachment process, cell membrane characteristics and cell-surface interactions is critical.

Keywords:

Microcarrier , Adhesion , Harvesting , Surface Modification , Tissue Engineering