Regulation of Stemness, Metastasis and Drug Resistance By MicroRNAs in Gastric cancer

Objective or background: Cancer stem cells (CSCs) have a significant role in spread out and cancer metastasis as well as resistance to conventional therapy. MiRNA is one of the regulatory elements which can regulate metastasis, stemness and drug-resistance properties simultaneously. Therefore, in this study, by using literature learning and data mining, 30 miRNAs obtained that are able to regulate any third of these properties in gastric cancer. Materials & methods: published articles from PubMed online database were obtained using search strategy:((stem cell OR sphere OR side population) AND (miRNA OR microRNA) AND (profiling)). 138 studies were collected from mentioned search strategy and after removing duplicates, non-full-text and non-related topics and abstracts, 21 studies were selected (figure 1). Figure 1. Flowchart of study selection.A total of 200 miRNAs which regulate stemness, were observed in 21 studies.For extracting miRNAs that regulate metastasis, we used the miRCancer database. Then we observed 76 miRNAs are common between metastasis and stemness.And for the drug resistance feature, we used the PUBMED database and analyzed 30 common miRNAs in these three properties. (figure 2).2. Venn diagram shows the Number of common miRNAs.RESULTS: Based on different sets of data, 30 miRNAs including: hsa-miR-197, hsa-let-7f, hsa-miR-34a, hsa-miR-21 , hsa-miR-7g , hsa-miR-107, hsa- miR-7d , hsa-miR-149 , hsa-miR-375 , hsa-miR-30a , hsa-miR-103a , hsa-miR-224 , hsa-miR-129-1, hsa-miR-197, hsa-miR-125b, hsa-miR-24, hsa-miR-20a, hsa-miR-223, hsa-miR-129-2, hsa-miR-149, hsa-miR-23b, hsa-miR-27a, hsa-miR-338, hsa-miR-181b, hsa-miR-27b, hsa-miR-140, hsa- miR-19b, hsa-miR-92, hsa-miR-524 and hsa-miR-106b play a role in resistance to conventional therapy, stemness, metastasis in gastric cancer.