The study of non coding RNA HOXD-AS1 gene expression in women with ovarian cancer

The study of non coding RNA HOXD-AS1 gene expression in women with ovarian cancerHdis karimi –saraskanrud1,Mohammad Khalaj-kondori2,*1MSc. of Genetics, Faculty of Science, Science and Research Branch, Islamic Azad University of Tabriz2Associate Professor of Molecular Genetics, Department of Animal Sciences, Faculty of Natural Sciences, University of Tabriz.* Corresponding author: [e-mail]: Ovarian cancer is a neoplasm in women and shows malignant behavior and poor prognosis. Current methods used for its diagnosis and prognosis, are not so useful. Studies have shown that the expression of lncRNA HOXD-AS1 gene is altered in cancers. This study aimed to investigate the expression pattern of HOXD-AS1 gene in ovarian cancer. Methods: Total RNA was extracted by RNX-Plus solution from tumoral and marginal tissue samples of 30 patients with ovarian cancer, and reverse transcripted by cDNA synthesis kit from TAKARA. Then, the HOXD-AS1 gene expression was measured quantitatively by Real-time PCR. Results and Discussion: The results revealed HOXD-AS1 gene expression in ovary and showed that its expression in tumoral tissues is altered compared to tumor marginal tissues. So, evaluation of its expression might be helpful in the diagnosis and prognosis of ovarian cancer.

Keywords:

ovarian cancer , HOXD-AS1 , lncRNA