Molecular epidemiology of colistin-resistant Pseudomonas aeruginosa producing NDM-1 from hospitalized patients in Iran
Publish place: The 19th Annual Congress of Medical Students of Iran
Publish Year: 1397
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
AMSMED19_274
تاریخ نمایه سازی: 1 دی 1397
Abstract:
Background and Objective: Resistance to carbapenems is the principal reason for the continuing utilization of colistin as a lastresort choice for treating the infections resulted from multidrug carbapenem-resistant Pseudomonas aeruginosa (CRPA) isolates. The assessment of antimicrobial resistance pattern, the prevalence of carbapenem-resistance determinants, and molecular epidemiology of colistin-resistant isolates among CRPA strains were the aims of the present research. Materials and Methods: The current cross-sectional research was conducted on 269 carbapenem-resistantP. Aeruginosa isolates collected from various clinical samples from 2013 to 2016. After performingidentification tests, disk diffusion as well as MIC methods were used for testing sensitivity to the antibiotics. Modified Hodge Test (MHT) was utilized to produce carbapenemase. PCR technique identified beta-lactamaseclasses A, B, and D genes. Findings: In total, from 269 CRPA, five isolates (1.3%) were resistant to colistin. It was found that blaNDM-1, blaIMP-1, blaVIM-2, and blaOXA-10 geneswere present in 40%, 40%, 20%, and 100% of colistin-resistant isolates, respectively. DLST type 25-11 is a significant cluster of colistin-resistant Pseudomonas aeruginosa isolates. Conclusions: The appearance of colistin-resistant isolates in CRPA carrying blaNDM-1 with multiple carbapenem-resistant genes shows the great problem in the treatment of P.aeruginosa infections.
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Authors
Ahmad Farajzadeh Sheikh
Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Mojtaba Shahin
Health Research Institute, Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Fahimeh Ghanbari
Department of molecular medicine, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran