Publisher of Iranian Journals and Conference Proceedings

Please waite ..
CIVILICAWe Respect the Science
Publisher of Iranian Journals and Conference Proceedings
عنوان
Paper

General identification and isolation of obligate anaerobic bacteria in patients with periodontitis and its association with genomic polymorphism of TGF_β1 beta using Tetra _ARMs_PCR

تعداد صفحات: 1 | تعداد نمایش خلاصه: 152 | نظرات: 0
سال انتشار: 1397
کد COI Paper: ICCM12_030
زبان Paper: Englishglish
نسخه کامل Paper در کنفرانس ارائه نشده است و در دسترس نیست.

مشخصات نویسندگان Paper General identification and isolation of obligate anaerobic bacteria in patients with periodontitis and its association with genomic polymorphism of TGF_β1 beta using Tetra _ARMs_PCR

Mojgan Sarabi nobakht - Master of Microbiology,Islamic Azad University ,Sirjan,Iran
Nooshin Khandan dezfoli - Assistant professor,Department of Microbiology,Sirjan Branch,Islamic Azad University,Sirjan,Iran
Kumarss Amini - Assistant professor,Department of Microbiology,Sirjan Branch,Islamic Azad University,Sirjan,Iran

چکیده Paper:

Introduction: Periodontitis is a common infectious disease that destroys the tissues that hold the tooth and subsequently loosen and tooth loss. Periodontal diseases are multi-microbial and multifactorial diseases and many factors in the host play a role in determining the susceptibility of people to the disease. The most important anaerobic bacteria involved in the periodontitis infection are: Porphyro-monas gingivialis, Proteella intermedia, Prevotella melanino genica, Fosubacterium nucleatum which can be detected and detected by biochemical methods. TGF-β1 is one of the growth factors and an anti-inflammatory cytokine that plays a decisive role in the repair of periodontal lesions. Considering the clinical importance of this type of infections and its complications, this study was conducted.Materials and Methods: This case-control study was performed by approving the University Ethics Committee and informed written consent in the periodontal department of Tehran Dental School. The sample size was 100 patients, including 50 healthy subjects and 50 patients the periodontal microbial infection was done by sampling. Anaerobic bacteria were identified using anaerobic bacteria culture method (anaerobic gas and jar) and extracted DNA from Tetra_arms_PCR patients‟ blood to determine the association between genomic polymorphism TGF_β1 and anemia Periodontitis is determined.Results: All samples were positive culture. More than 65% of the isolated anaerobic bacteria were obligate: Prevotella, Porphyro-monas, Fosubacterium , Peptostreptococcus anaerobious and results from Tetra_ARMs_PCR. After performing the sequencing and reviewing with SNPP software, the results were observed. Frequency of genotype CC, TT CT was 25, 20 and 5%, and the percentage of control group was 20,24and 6%, the frequency of C and T allele in the patient group was 30 and 70%, and in the control group 64 and 36, there was no significant difference in the frequency of alleles and genotypes between the two groups.Conclusion: there is a significant relationship between polymorphism There is no genotype TGF_β1 and periodontitis.

کلیدواژه ها:

Periodontal, TGF_β1, Tera arms_PCR, Obligate anaerobic bacteria

کد Paper/لینک ثابت به این Paper

برای لینک دهی به این Paper می توانید از لینک زیر استفاده نمایید. این لینک همیشه ثابت است و به عنوان سند ثبت Paper در مرجع سیویلیکا مورد استفاده قرار میگیرد:

https://civilica.com/doc/810875/

کد COI Paper: ICCM12_030

نحوه استناد به Paper:

در صورتی که می خواهید در اثر پژوهشی خود به این Paper ارجاع دهید، به سادگی می توانید از عبارت زیر در بخش منابع و مراجع استفاده نمایید:
undefined, undefined و undefined, undefined و undefined, undefined,1397,General identification and isolation of obligate anaerobic bacteria in patients with periodontitis and its association with genomic polymorphism of TGF_β1 beta using Tetra _ARMs_PCR,دوازدهمین کنگره بین المللی میکروب شناسی بالینی ایران,Kerman,,,https://civilica.com/doc/810875

در داخل متن نیز هر جا که به عبارت و یا دستاوردی از این Paper اشاره شود پس از ذکر مطلب، در داخل پارانتز، مشخصات زیر نوشته می شود.
برای بار اول: (1397, Sarabi nobakht, Mojgan؛ Nooshin Khandan dezfoli و Kumarss Amini)
برای بار دوم به بعد: (1397, Sarabi nobakht؛ Khandan dezfoli و Amini)
برای آشنایی کامل با نحوه مرجع نویسی لطفا بخش راهنمای سیویلیکا (مرجع دهی) را ملاحظه نمایید.
Support