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SAP3 gene expression in Candida albicans strains isolated from Vulvovaginal Candidiasis

تعداد صفحات: 1 | تعداد نمایش خلاصه: 118 | نظرات: 0
سال انتشار: 1397
کد COI Paper: ICCM12_143
زبان Paper: Englishglish
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مشخصات نویسندگان Paper SAP3 gene expression in Candida albicans strains isolated from Vulvovaginal Candidiasis

Ahmad Jabrodini - Department of Laboratory Sciences, School of Paramedical, Gerash University of Medical Sciences, Gerash, Iran
Seyedeh Faezeh Taghavi - Department of Laboratory Sciences, School of Paramedical, Gerash University of Medical Sciences, Gerash, Iran
Elmira Zarei - Laboratory Science students, Gerash Student Research Committee, Gerash University of medical sciences, Gerash, Iran

چکیده Paper:

Introduction and Objectives: Candida albicans is the main cause of Vulvovaginal Candidiasis (VVC). These yeasts produce Secreted aspartyl proteinases (Saps) encoded by a family of 10 genes (SAP1-SAP10). The Sap3 enzyme is effective in the initial development of VVC due to its158role in the adhesion and colonization of Candida albicans. The aim of this study was to evaluation the expression of SAP3 gene in Candida albicans strains isolated from VVC.Material and Methods: Vaginal secretion samples were collected from 268 non-pregnant vaginitis patients referred to Amiralmomenin Hospital of Gerash from January to July 2018. All samples were examined under direct microscopy and cultured on Sabouraud dextrose agar medium with chloramphenicol (50mg/L). Candida species were identified by using standard phenotypic and sugar assimilation test (API20C). Proteinase activity was determined by using YCB-BSA medium. Genomic DNA extraction was done by use of chloroform-phenol-isoamyl alcohol method. PCR method was used for the presence of SAP3 gene. Chi-square test was used for data analysis.Result: Out of 268 samples, 79 cases (29.47%) were positive for Candida species, among them 48 candida albicans strains (60.75%), 16 Candida glabrata strains (20.25%), 12 Candida parapsilosis strains (15.18%) and 3 Candida tropicalis strains (3.79%) were isolated. The average of proteinase activity in candida albicans strains was 0.26 ± 0.08 (Unit/106 cells/ml, ±SD). The results of PCR showed the presence of SAP3 gene in 47 Candida albicans strains (97.91%). There is not significant relation between proteinase activity and SAP3 gene presence (P> 0.05).Conclusion: The results showed that Candida albicans was more likely to cause Vulvovaginal than other Candida species. The reason is the presence of the SAP3 gene in most strains of Candida albicans and their high proteinase activity compared to other Candida species.

کلیدواژه ها:

Candida albicans, SAP3 expression, Vulvovaginal Candidiasis, PCR

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https://civilica.com/doc/810988/

کد COI Paper: ICCM12_143

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undefined, undefined و undefined, undefined و undefined, undefined,1397,SAP3 gene expression in Candida albicans strains isolated from Vulvovaginal Candidiasis,دوازدهمین کنگره بین المللی میکروب شناسی بالینی ایران,Kerman,,,https://civilica.com/doc/810988

در داخل متن نیز هر جا که به عبارت و یا دستاوردی از این Paper اشاره شود پس از ذکر مطلب، در داخل پارانتز، مشخصات زیر نوشته می شود.
برای بار اول: (1397, Jabrodini, Ahmad؛ Seyedeh Faezeh Taghavi و Elmira Zarei)
برای بار دوم به بعد: (1397, Jabrodini؛ Taghavi و Zarei)
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