Immunophenotyping of 184 pationts with AML in shahid bahonar hospital (2012_2018)

Introduction. In view of the pitfalls in FAB (French American British) classification which was based on morphology, theWHO classification emphasized the importance ofimmunophenotyping and defined the myeloid and lymphoid malignancies by the antigenic features of the neoplastic cells. Flowcytometry (FCA) is a powerful technique by whichsuch antigens can be identified. Identifying the lineage of the leukemic cells not only helps in assessing the course of the disease but also aids in rendering the most specific treatment to the patients. In this report, our objective is to investigate the expression of the membrane-differentiation antigens CD13, CD15, CD33, and CD34 in 184 adult patients with newly diagnosed AML. Methods: Bone marrow samples were obtained from 184 adult patients AML (2012-2016) diagnosed by using morphology and a panel of monoclonal antibodies by multiparametric flowcytometry. Immunophenotyping was done on fresh bone marrow aspirate using the MoAbs is stained with Phycoerythrin (PE) /fluorescein isothiocyanate (FITC). Results: Out of the184 cases with AML 102 (56.6%) cases were male and 78 (43.3%) of them were female. CD34 was positive in all of cases in M1, M4, M5, M6 and 95.4% in M2. We detected CD117 in all of M1, M5, M6 cases, 95.40% of M2 cases, 96.8 of M3 and 91.6% of M4. Of 184 patients were 141 cases HLA-DR positive (76.6%) and 34 casesHLA-DR negative (18.4%). HLA-DR was positive in all of cases in M2, M6 and 95% in M1, 93.7% in M3, 98.3% in M4 and 88.8% in M5. HLA-DR was negative 5% in M1,6.3% in M3, 1.7% in M4 and 11.2% in M5.Conclusions: HLA-DR was positive in all of cases inM0 M2, M6 and 95% in M1, 93.7% in M3, 98.3% in M4 and 88.8% in M5. HLA-DR was negative 5% in M1, 6.3% in M3, 1.7% in M4 and 11.2% in M5.

Keywords:

immunophenotyping , AML , WHO classification