Samaneh Soltani - Department of Materials Engineering, Isfahan University of Technology, Isfahan, Iran
Rahmatollah Emadi - Department of Materials Engineering, Isfahan University of Technology, Isfahan, Iran
Shaghayegh Haghjoo Javanmard - Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran
Mahshid Kharaziha - Department of Materials Engineering, Isfahan University of Technology, Isfahan, Iran

Background and Aim: Injectable hydrogels afford direct injection orcatheter delivery to tissues. However, many injectable hydrogels requirelong reassembly times or the use of triggers such as chemical initiatorsor heat.Methods: We developed an injectable hyaluronic acid (HA) hydrogelbased on the guest−host interactions of adamantine modified HA (guestmacromer, Ad-HA) and β-cyclodextrin modified HA (host macromer,CD-HA).Results: The ability of conjugation to HA was confirmed by 1H NMRspectroscopy. Mixing of Ad-HA and CD-HA resulted in rapid formationof a hydrogel composed of guest−host bonds.Conclusion: The guest−host assembly mechanism allowed both shearthinningbehavior for ease of injection and near instantaneous reassemblyfor material retention at the target site. These hydrogels show potential asa minimally invasive injectable hydrogel for biomedical applications andtissue engineering.

Shear thinning hydrogels; Hyaluronic acid; Graphene oxide; Cardiovascular tissue engineering