Introduction of a simple chemical protocol for induction of type 2 diabetes in mice

The type 2 diabetes occurs in both human and animal models when beta cell function and/or mass are impaired to the extent that insulin production is not sufficient to compensate for insulin resistance. As obesity-induced insulin resistance is a major contributor to the pathogenesis of Type 2 diabetes, many animal models areeither genetically obese or obesity is induced by manipulation of the diet. Therefore, there are complex methods to induce type 2 diabetes such as multiple lowdosestreptozotocin along with high fat, high sucrose diets protocols administration and other combined methods which induce modest DNA damage, leading to the incomplete destruction of β cells for insulin-resistant diabetes in mice. In this experimental study, an innovative and simple protocol was investigated to induce type 2 diabetes by only Incremental Multiple Low-Doses Streptozotocin (IMLDS) in mice. IMLDS designed protocol was conducted in 4 consecutive days by streptozotocin injections of 20, 40, 80 and 160 mg/kg BW/day, respectively. The glucose, insulin, glucagon-like peptide 1 (GLP-1), RAC-beta serine/threonine-protein kinase and dipeptidyl peptidase IV (DPP-4) of blood were assessed by ELISA. The glucose, GLP-1, RAC-beta serine/threonine-protein kinase levels, and food and water intake increased (p<0.01) while serum DPP-4 activity decreased (p<0.01) after the administration of novel protocol. Also, by executing this protocol, the serum insulin increased (p<0.05). This new protocol may providea practical and unique alternative chemical method for the induction of insulin resistance diabetes (type 2 diabetes) in this animal model. Additional researches in this area are continues.

Keywords:

AKT-2 , Diabetes , DPP4 , GLP-1 , Insulin