Study of expression of 14.3.3 zeta protein gene in lung tissue in hypothyroidism male rats

Thyroid diseases are one of the most common diseases. Since the lung is one of the main target tissues for thyroid hormones. There are significant changes in the function of the lung in thyroid diseases. So, determining biomarkers for thyroid diseases is important. 14.3.3 proteins are a group of highly conserved proteins that are involved in many cellular processes. Two of the isoforms of this protein that have been reported in lung tissue are zeta and epsilon. Here, the effect of thyroid hormone on 14.3.3z expression in lung tissue was investigated. Samples were isolated from thirty Wistar adult male rat (weighting 250±50g) that were divided into three groups control (intact), hypothyroid (6-Propyl-2-thiouracil (PTU) 0.05g/l for 8 weeks) and treatment (Levothyroxine 0.2g/l for 8 weeks). Total RAN was extracted by using RNX-plus reagent, cDNA was synthesized by random hexamer primer RT reagent kit (Takara). Expression of 14.3.3z was evaluated by qRT-PCR. GAPDH was used as an internal control gene. The relative expression of 14.3.3z showed increased expression in the treated group compared to control group (p<0.05). There was not a significant change in the hypothyroidism group compared to the control group (P> 0.05). The results showed that exogenous injection of thyroid hormones leads to changes in expression 14.3.3z protein and role of this isoform in the signaling pathway thyroid hormones of the respiratory system. However, more studies with a high number of specimens are needed to make sure.

Keywords:

Thyroid hormones , Lung , 14.3.3 proteins , qRT-PCR , Cell signaling