Preparation, evaluation and characterization of biomimetic DBM scaffold

Bone tissue engineering is a combination of 3D, threedimensional, scaffolds and cells for repairing damaged or defective bone tissue. Therefore, extracellular matrix (ECM) derived scaffolds are a useful tool for providing a (micro) Environment for precisely evaluation of cellular interactions in 3D. The prepared fresh cow bones were converted into small pieces, and Freeze dried after physical and chemical decellularization. After processing by mixer mill under liquid nitrogen, derived nano powdr, were used for production of the DBM derived biomimetic electrospun nanofibrous scaffolds. Radiographic methods, electrolyte analysis and calcium oxalate tests were used to prove calcification. The nanofibrous scaffolds have been verified for surface properties, biodegradability and porosity. The results from the scanning electron microscopy, revealed the appropriate morphology and surface properties of them. After the biocompatibility evaluation of scaffolds for the human umbilical cord derived mesenchymal stem cells, the cells growth, proliferation and adhesion to the scaffold were confirmed using cytotoxicity evaluation via MTT-assay and microscopic studies. Therefore, this biomimetic scaffold may be used as a suitable candidate in regenerative medicine and bone tissue engineering.

Keywords:

Nanofibrous scaffolds , DBM , Biomimetic , Stem Cell , Biocompatibility , MTT