Investigation of the preventive effect of Rivastigmine and aqueous extract of Olibanum in Alzheimer s disease

Alzheimer s disease (AD) is a multifactorial disorder that it´s progression is associated with many genetic and environmental factors. Many studies showed that the FMR1gene is important in the AD because FMR1 protein is participated in regulating of APP production. One of the most common plants to improve memory performance is the Boswellia resinate. It seems that the resin of Boswellia considerably can affect brain development and correct formation of neuritis (axon and dendrites). So, we investigated the Preventive effects of aqueous extract of Olibanum and Rivastigmine on the expression of the FMR1 gene in rats that treated with AlCl3. 28 adult male rats randomly divided into 4 groups (n=7). Group 1 was treated with distilled water (1 ml), group 2 with AlCl3 (0.3 mg/kg), group 3 with aqueous extract of Olibanum(200mg/kg), Group 4 was treated with Rivastigmine(0.3 mg/kg),all the Group treated with our own substance for 14 days Then treated with AlCl3(20 mg/kg) for 56 days(Except control Group). Animals were tested with Morris-Water- Maze and their hippocampus was isolated and used for FMR1 gene expression analysis by Real-time PCR. Total RNA was extracted by using RNX-plus solution according to the manufacturer’s protocol. Then, cDNA was synthesized by PrimeScriptTM RT reagent Kit (TaKaRa). GAPDH was used as an internal control gene. Morris- Water-Maze test showed that AlCl3 impairs memory performance in rats and Real-time PCR data analysis indicated that while AlCl3 led to significant reduction in FMR1 gene expression (0.018), Rivastigmine increased its expression (0.0675) but an aqueous extract of Olibanum no significant effect (0.021).

Keywords:

Rivastigmine , Olibanum , FMR1 , Alzheimer’s disease