Investigation of the hypoglycemic, antioxidant and anti-bacterial effects of the waste of four rice varieties in Gilan province

The growing interest on the replacement of synthetic antioxidants with natural ones has directed many researches toward the plant-derived raw materials. The special attention is focused on inexpensive and residual sources from food agricultural industries. In the present study, the antioxidant, anti-bacterial and anti-amylase properties of the acetone extract of the bran of four rice varieties of Guilan province, including Hashemi, Tarom, Neda and Dasht were investigated. Anti-amylase activity was evaluated by Bernfeld method using DNS reagent. DPPH and FRAP methods were used to determine the antioxidant activity of the extract. Also, total phenol and flavonoid content and anthocyanin were also estimated. Folin and ciocalteu methods were used to determine the total phenol. Colorimetric aluminium chloride methods were used to determine flavonoid and mita et al methods were used to determine anthocyanin. Antibacterial properties were studied by disk diffusion method. The results showed that the highest ferric reducing ability belongs to acetone extract of Dasht wastes (1/36 mM Fe (II)/g DW) and the highest percentage of free radical inhibitory belong to the acetone extract of Neda variety. Also, the highest amounts of phenol (0.79 mg GAE/g DW) flavonoids (55.49 μg QE/g DW) and anthocyanins (0.016mg/g DW) belong to the acetone extract of Dasht variety. Tarom variety showed the most efficient antibacterial activity on Escherichia coli and Micrococcus luteus bacteria, whereas Neda and Dasht varieties showed anti-bacterial potential against Pseudomonas Aeruginosa and on Staphylococcus aureus.The extracts were prepared using mixed solvent possessed hypoglycemic activity

Keywords:

Rice waste , Hypoglycemic , Antioxidant , Antibacterial