Mohadesseh Asadi, - Department of Biology, Faculty of Science, University of Tehran, Iran
Neda Karami, - Department of Biology, Faculty of Science, University of Tehran, Iran
Ehsan Azin - Department of Biology, Faculty of Science, University of Tehran, Iran

Impranil as one of the main polymer components in plastics manufacturing is one of the degradation resistance pollutants in the environment. Serious health problems caused by these chemicals have highlighted developing the efficient degradation methods. Therefore, this project was aimed at isolation of impranil consuming microorganisms as the sole source of carbon .Four soil samples were collected from hydrocarbon contaminated regions and transported to the laboratory under standard condition. Isolation of capable microorganism in using impranil as the carbon source was conducted in M9 minimal salt medium with 1% impranil as the sole source of carbon. Evaluation of clear zone formation on the M9 medium as an indicator of impranil degradation capability confirmed the growth of one fungal isolate in the presence of 1% impranil. Microscopic and macroscopic characterization of the selected isolated showed that the fungi belong to Aspergillus genus. The fungi were cultured in M9 medium with 2.5%, 5%, 7.5% and 10% NaCl in the presence of 1% impranil in three replicates. At the end of incubation period, the fungi showed to tolerate the salt up to 7.5%. The further analysis should be performed to determine the capacity of the fungal isolate in impranil removal.

Impranil, M9 medium, Aspergillus sp., Plastic degradator