Isolation of immunodominant proteins of Naja Naja (oxiana) snake venom
Publish Year: 1397
نوع سند: مقاله کنفرانسی
زبان: English
View: 348
نسخه کامل این Paper ارائه نشده است و در دسترس نمی باشد
- Certificate
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
BIOCONF20_370
تاریخ نمایه سازی: 28 اردیبهشت 1398
Abstract:
Snake venom is a complex mixture of proteins, peptides, enzymes, carbohydrate, and mineral. Snake venom contains a variety of chemicals including pharmacological and toxicological properties. The innate immune system is the first line of defense against toxin and microbe. The aim of the present study is to investigate and isolate immunodominant proteins of Naja oxiana snake venom. Identification was performed by SDS-PAGE 15% and western blot analysis. Subsequently, four sharp protein bonds 14, 22, 32, 64 kDa, were appeared in nitrocellulose paper. The next step identified proteins were isolated directly by Electro-elution from preparative gel electrophoresis. To the best our knowledge, these proteins may be a candidate for specific antivenom or antiserum against Naja oxiana
Keywords:
Authors
Mahboobeh Talebi Mehrdar
Department of science, Faculty of biochemistry, University of Payame noor, Tehran, Iran