Study of ct-DNA interaction with celecoxib (celberx)

In this paper, the interaction of celcioxide with cytomegalovirus DNA (DNA) using ultraviolet-visible spectroscopy, fluorescence spectroscopy, electrophoresis, infrared spectroscopy, molecular docking, in different concentrations of ct-DNA and celecoxib, with the goal of designing more effective countermeasures Cancer has been studied with low side effects. The visible UV-absorbance spectrum of celecoxib showed an effect of hyperchromicity in the presence of DNA, and a constant binding of cecoxib with a DNA of 2/7 ×104 was estimated. The results show that celecoxib bonded to the DNA through the groove (connection of the groove type).All of the above experimental techniques confirmed the results of visible-ultraviolet absorption spectroscopy in order to detect the interaction of cell coccyb with the type of groove (large or small) of Docking studies. The results of this study, in addition to confirming the experimental results, gave the type of interaction The action was determined through a small groove with high affinity with guanine cytosine.

Keywords:

Interaction , Celecoxib , DNA of calf thymus , spectroscopy , molecular docking