Binding interaction of perphenazine with calf thymus DNA: a spectroscopic and molecular docking study

In this work, study the interaction of ct-DNA (calf thymus DNA), as a main intracellular target for various small molecules, with perphenazine (PP), an antipsychotic agent, was performed using different techniques including fluorescence spectroscopy, UV-vis absorption spectroscopy, viscometry measurement and molecular docking studies. UV–vis absorption spectroscopy results revealed that the interaction of PP with ct-DNA is a groove binding mode and the binding constant (Kb) value was calculated to be 8 × 103 M-1. Competitive fluorimetric studies with methylene blue (MB) displayed that PP possibly fits into the minor groove of ct-DNA. The results of viscometric studies indicated that no significant change occurs in the length of ct-DNA and confirm the groove binding mode of PP with ct-DNA. All experimental results were confirmed by molecular docking outputs

Keywords:

Perphenazine , Groove binding , Molecular docking , Calf thymus DNA