Alpha-glucosidase inhibitory activity by hexane extract from different aerial parts of plants, Haplophyllum acutifolium DC. and Ferula haussknechtii Wolff ex Rech

The dietary carbohydrates should first be broken down to monosaccharides by some gastrointestinal enzymes since only monosaccharides can be absorbed from the intestinal lumen. α-Glucosidase is the key enzymes involved in the digestion of carbohydrates. Liberated glucose is then absorbed by the gut and results in postprandial hyperglycemia. The control of postprandial hyperglycemia is an important strategy in the management of diabetes mellitus. Since the effect of hexane extracts of Haplophyllum acutifolium DC. and Ferula haussknechtii Wolff ex Rech has already been demonstrated, the purpose of this study is to investigate and identify organs of theabove plants that show maximum inhibitory activity, as well as the measurement of the antioxidant activity of these organs. Hexane extracts of isolated organs of theplants were prepared using a rotary evaporator. The inhibitory effects of the extracts were investigated in six concentrations at 405 nm wavelength using a microplate reader. Antioxidant activity of the extracts was measured using the DPPH scavenging test. The highest inhibitory activity of Ferula haussknechtii Wolff ex Rechwas observed at the 100 mg/ml concentration (100% inhibition and IC50= 0.0001mg/ml). The most inhibitory activity ofHaplophyllum acutifolium DC.was related to a 1000 mg/ml concentrationflower organs(100% inhibition and IC50 = 0.01 mg/ml) and leaf. (100% inhibition and IC50 = 0.06 mg/ml). Haplophyllum acutifolium DC.organs have antioxidant activity of 100% and EC50 = 6.21mg/ml, EC50 = 2.37mg/ml for flower and leaf respectively. Ferula haussknechtii Wolff ex Rech leaves and stem also have 100% antioxidant activity and EC50 = 0.96 mg/ml EC50 =22.4 mg/ml respectively. The results of this study indicate that the hexane extract of these two organs possesses effective inhibitors, and the attempt to isolate these materials and their interactions with the enzyme is a good subject for future research with the aim of accessing inhibitors with pharmaceutical uses.

Keywords:

α-Glucosidase , Inhibitor , Hexane extract , Haplophyllum acutifolium , Ferula haussknechtii