Investigation of covalent attachments between functionalized tungsten disulfide and anti-Hepatitis B antibody

Hepatitis B can cause two types of acute and chronic illness and cause damage to the liver and even death. So the early diagnosis of this virus is very effective. To enhance the signals and to increase the sensitivity and detection of hepatitis B viruses, the active tungsten disulfide mineral nanotubes are used.Tungsten disulfide nanoparticles (INTs-WS2) are used to polycarboxylate or polyaminoisation the surfaces of these nanotubes, which is responsible for the solubility of these nanotubes in conventional solvents.In fact, PolyCoH f -INT-WS2 was created by polycarboxylation, which uses the Vilsmeier-Haack electrophilic property (VH). In this method, the mixture containing DMF (secondary N-formyl amine), Oalkylating 2-Bromo-acetic acid (2-BrCH2COOH), and silver acetate (Ag (L) OAc) as a bromophilic agent to enhance abstracting the halogen from bromo acetic acid, is used for polycarboxylation of INT-WS2. The DMF and the ionization of silver bromophyll cations are very necessary, and their absence prevents polycarboxylation. In this test, the binding of anti-hepatitis B virus antibody to the functionalized WS2 nanotubes was performed by creating a covalent bond between carboxyls at the surface of the WS2 nanotubes functionalized, and a surface amine of lysine or arginine of anti-hepatitis B virus antibodieswas conducted. This method can be a useful and effective way to detect the hepatitis B virus faster and help people with the disease.

Keywords:

Functionalized tungsten disulfide , Anti-Hepatitis B antibody , covalent attachment