Study of the interaction between Nelfinavir and complex (DNA-H1) bymultispectroscopic techniques

The interaction of small molecules with DNA plays an important role in many biological processes .DNA binding studies have remarkable relevance and implications in drug design aspects and cancer chemotherapy. Histones act as spools around which DNA winds. This enables the compaction necessary to fit the large genomes of eukaryotes inside cell nuclei .Histones undergo posttranslational modifications that alter their interaction with DNA and nuclear proteins. Nelfinavir (brand name Viracept) is an antiretroviral drug used in the treatment of the human immunodeficiency virus (HIV). Nelfinavir belongs to the class of drugs known as protease inhibitors (PIs) and like other PIs is almost always used in combination with other antiretroviral drugs .Its chemical formula is CH32H45N5O4S and its molecular weight is 568.784 g / mol. The interaction between complex (DNAH1) and Nelfinavir it has been studied by using different spectroscopic techniques vis., resonance light scattering (RLS) spectra and circular dichroism (CD) spectra, in physiological buffer pH=6.8. The RLS method determined the critical aggregation concentration of drug on complex (DNA-H1). When nelfinavir was added to (DNA-H1) complex, the RLS intensity of the system was increased. It is known that RLS enhancement could be the result of the enlargement of the molecular volume and large size of the complex Circular dichroism is a suitable method for monitoring the secondary and tertiary structure of proteins. Quantitative analyses of the CD spectra of the interaction between (DNA-H1) and drugs secondary structure. Change of the secondary structure of DNA-H1 involving an increase of α-helix and chirality has increased Electrostatic forces and hydrogen bonds.

Keywords:

Nelfinavir , Complex-DNA-H1 , RLS , CD