Studies on the interaction of the drug Nelfinavir with calf thymus DNA by resonance light scattering and circular dichroism spectroscopy

The DNA molecule carries all the information necessary to form the physical properties of the individual, and these properties are essentially determined by the protein. Therefore, DNA contains instructions for making a protein. In fact, DNA has included in its structure the information that inherits the living entities of its predecessors. A slight change in the structure of DNA may have serious consequences and, if it is degraded to an irreversible extent, causes the death of the cell. Nelfinavir is an antiretroviral agent that, as an HIV protease inhibitor, can inhibit both type 1 and type 2 HIV protease. Usually, this drug is used to treat HIV infection with a reverse transcriptase inhibitor. In this study, the interaction between Nelfinavir drug and calf thymus DNA at ambient temperature and pH= 6.8 was investigated by resonance light scattering and circular dichroism spectroscopy. The resonance light scattering results showed a change in the macromolecule structure, indicating the formation of a complex between the nelfinavir and the calf thymus DNA. The chart Critical Point of Creation Sediment on the nelfinavir concentrations emphasized the formation of the complex between the drug and the DNA. Further, it showed that the drug in the lower concentrations could also cause changes in the DNA structure. cd spectroscopy was used to investigate the structural changes in DNA in interaction with nelfinavir. Spectrometry results show that the interaction of nelfinavir with DNA leads to a change in the structure of the second macromolecule. Also, according to the results obtained, it is likely that this interaction occurs between the pair portions of DNA.

Keywords:

Nelfinavir , DNA , Resonance light scattering , Circular dichroism