Evaluation of methylation and expression of miR-96 in tumor tissue versus margin in patients with breast cancer

Breast cancer and its metastatic progression are mainly driven by changes in epithelial to mesenchymal state, a phenomenon that relies on specific transcription agents and miRNAs. MicroRNAs, as key regulators of the expression of genes after transcription, can participate in the control of physiologic and pathologic cellular processes. Given that a microRNA can act as an oncogene or tumor suppressor and can also control the expression of several genes, the change in the methylation pattern of the promoter region of the gene encoding it in the microRNA and as a result of its expression change can lead to various cancers. Several microRNAs have been studied in a variety of cancers. This study examines miR-96, which is related to the conserved miR cluster of 183/182/96. In this study, the tissue samples extracted from the Tumor tissue and its margin of 100 patients with breast cancer were crushed using liquid nitrogen and the DNA samples were extracted manually and the RNA sample was extracted using a kit. Then, cDNA of this samples were synthesized and performed RT-PCR and the results were analyzed, there was a significant relationship between the expression and the methylation of this microRNA in breast cancer. The results showed that miR-96 had a significant upregulation in tumor tissue in breast cancer due to hypomethylation.

Keywords:

Cancer , Methylation , Expression , microRNA