Evaluation of methylation and expression of miR-196b in tumor tissue versus margin patients with breast cancer

There are many indications that the change in the methylation pattern of the promoter region of the miRs encoding genes is the most epigenetic change observed in cancers. Previous studies have shown that miR-196b act as an oncogene or tumor suppressorin various cancers. The aim of this study was to investigate the role of this miR in breast cancer as one of the most common cancers among women and the effect of methylone on its expression. In this research, by evaluating the expression levels of miR-196b extracted from 100 tumor tissue and tumor margins by Real-time PCR, and evaluating the methylation of the mir-196b gene promoter by treating DNA samples with sodium bisulphate and performing MS-PCR and comparing the methylation state of this miR with Its expression in the tumor tissues relative to its margins was shown that have a significant relationship between the aberrant methylation of the promoter region and its expression level. As a result, its alteration of expression patterns could be involved in the process of invasion of breast cancer into secondary tissues and convert this miR to a Diagnostic biomarker in breast cancer and as one of the therapeutic goals of this cancer, Should be considered.

Keywords:

Expression , Methylation , microRNA , Cancer , Invasion