DSCAM-AS1 lncRNA upregulates in ductal breast cancer tumoral tissues

Breast cancer as the most lethal malignancies among women in the world is a heterogeneous disease and its clinical management is difficult. So, determining biomarkers for diagnosis and prognosis of this cancer is important. Long non-coding RNAs (lncRNAs) are transcripts with more than 200bp in length that do not code any protein. They are an important category of RNAs involved in many important biological processes including cancer. One of the most novel lncRNAs that have been reported in cancer progression is DSCAM-AS1. Here we aimed at evaluation of the DSCAM-AS1 expression in ductal breast cancer. To test this hypothesis, tumor samples from 25 patients with breast tumor were collected (breast tumor tissues and their marginal normal ones). Total RNA was extracted using RNX-plus and cDNA was synthesized by PrimeScriptTM RT reagent kit (TaKaRa). Level of the DSCAM-AS1 lncRNA expression was measured using Real-Time PCR and the results compared between the two groups. GAPDH was used as an internalcontrol gene. We observed increased expression of the DSCAM-AS1 in tumor tissues compared with marginal ones (fold change: 5, p-value ≤ 0.05). In conclusion, this study further implicates DSCAM-AS1 lncRNA as a strong genetic candidate in breast cancer. However, more specimens might be analyzed

Keywords:

Ductal breast cancer , lncRNA , DSCAM-AS1 , qRT-PCR