Improvement the effect of green synthesized nano-oxali palladium in comparison with oxali palladiumagainst human colon cancer cell line HCT116

Previous reports showed the anti-cancer activity of oxali-Palladium and turmeric extract in colon cancer treatment. In this study, we investigated the cytotoxic effects of green synthesized nano-oxali palladium using turmeric extract by green chemistry method in comparison with free oxalipalladium against human colon cancer cell line of HCT116. At first, the nano-oxali palladium had to be synthesized by green chemistry method, then the cytotoxicity and antiproliferative activities of nano-oxali palladium, oxali palladium, and turmeric extract were examined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay after 24 and 48 hours incubation times. To synthesize of nano-oxali palladium, the alcoholic extract of turmeric incubated with an oxalipalladium solution at 500 C for 24 h in shaker incubator then physical and chemical properties of nano particle such as size, shape and etc.were studied. The 50% cytotoxic concentration (Cc50) of the nano-oxali palladium was calculated 78 and 57 (μM) after 24 and 48 h incubation times whereas this value was evaluated 600 and 433 μM for free oxali palladium after 24 and 48 h incubation times. Respectively, 45 and 32 (mg/ml) of turmeric extract after 24 and 48 h incubation times were induced death in 50% of HCT116 cell line. The results show that green synthesized nano-oxali palladium using turmeric extract has better or more cytotoxic effect on HCT116 colon cancer cell line to induce death in 50% of cells after different incubation times of 24 and 48 hours. Also, the above results illustrate that the anti-cancer activity of turmeric extract which is used as the cover of nanooxalipalladium is amplifying in nano form and increases the cytotoxicity of this compound.

Keywords:

Colon cancer , Oxali Palladium , Turmeric extract , Green chemistry