Designing, synthesizing and cloning of equine follicle stimulating hormone in prokaryotic host

Biotechnology is increasingly engaged in various fields of science and industry to create new products or improve existing conditions, and industry and animal science are no exception. One of the most important biotechnology approaches in this regard is the reproductive screening of livestock by biological means and processes for the production and regeneration of livestock with particular and prominent traits or increasing the proliferation of a particular type of one. This issue is important not only in terms of economics but also in aspects such as the survival of species at risk of destruction and the preservation and management of alleles and genes in the environment. In this study, after evaluating and optimizing the horse s follicular stimulus hormones gene (eFSH), the gene was synthesized in the puc57 vector that also possesses the antibiotic resistance gene of ampicillin. The vector was transferred to the host of the E. coli prokaryotic virus to propagate and after recombinant colonization was cultured on the selected medium. The vector was transferred to the prokaryotic host E. coli virusto propagate and after culturing on Selective culture medium the recombinant colonies was extracted. For these colonies, the molecular parameters of colony PCR were defined and performed,which confirmed the amplification of the desired gene.

Keywords:

Prokaryotic host , Cloning , eFSH