Mohammad Shokrzadeh - Pharmaceutical Sciences Research Center AND Department of Toxicology, School of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
Ali asghar Ahmadi - Fatemeh Zahra Infertility and Reproductive Health Research Center, BabolUniversity of Medical Sciences, Babol, Iran
Azadeh Kazemi - Genetic Department, Sana institute of Higher Education, sari, Iran

Nowadays, the researchers have taken promising steps to treat infertility using stem cell technology by differentiating stem cells, e.g. embryonic stem cells (ESCs), into male and female germ cells in vitro. The aim of this study is to examine the effect of human follicular fluid (hFF) on the differentiation of mouse ESCs. In this experimental study, transgenic OCT4-GFP ESC (Gift from Max-Planck-Institute) cultured with hFF alone (two concentrations: 25% and 15%) and along with retinoic acid at three times (7, 14, 21 days). The results showed that in all periods of time, hFF resulted in decrement of Mov10l1 expression in comparison with control group. The hFF 25% group reduced Mov10l1gene expression more than the hFF 15% group, in all three time periods studied. In combined groups, the expression of this gene is higher than those groups treated merely with hFF. hFF contains various compounds and hormones, can be effectively play a role in differentiation. The present study has shown that mouse ESCs could be differentiated in the presence of hFF, but there is no evidence to suggest that it should be male germ cells. However, the presence of retinoic acid is able to stimulate gene expression.

Follicular fluid, Stem Cells, Mov10L1, Gene Expression, Retinoic acid