Spermatogenic and phylogenetic characterizations of isolated fasciola sp. from natural host (cattle) in north west of iran

Fascioliasis is a parasitic disease that very important in livestock industry that caused with Fasciola hepatica or Fasciola gigantica with different hosts. The objective of this study was to identify these two species F. hepatica and F. gigantica based on spermatogenesis and using nuclear and mitochondrial genes (ITS1, ND1 and CO1) and have been employed to analyze intraspecific phylogenetic relations of Fasciola sp. Approximately 150 Fasciola specimens were collected from cattle and stained with haematoxylin-carmine dye and observed under an optical microscope to examine for the existence of sperm. The ITS1 marker was used to identify different Fasciola and phylogenetic analysis based on ND1 and CO1 sequence data were conducted by maximum likelihood algorithm. Fasciola samples were separated into 2 groups. Almost all specimens had many sperms in the seminal vesicle (spermic fluke) and one fluke did not contain any sperm in the seminal vesicle. The aspermic sample had F. gigantica RFLP pattern with ITS1 gene. Phylogenetic analysis based on NDI and COI sequence data were conducted by maximum likelihood showed a similar topology of the trees obtained particularly for F. hepatica and F. gigantica. This study demonstrated that aspermic Fasciola found in this region of Iran has same genetic structures through the spermic F. gigantica populations in accordance to phylogenetic tree

Keywords:

Fasciola , Phylogeny , North West , Iran