Determination of antibiotic resistance pattern and molecular diagnosis of β -lactamase genes (blaSHV, blaTEM, blaCTX-M) in Klebsiella pneumonia isolates from in-patient of Ilam hospitals

Extended-spectrum β-lactamases producing bacteria have spread widelythroughout the world. Production of these enzymes induces resistance to a wide range ofantibiotics in bacteria, which leads to a limitation of infection control and correct treatment. The aim of thepresent study was to investigate the antibiotic susceptibility of Klebsiella pneumonia isolates to beta-lactams antibiotics and presenceof β-lactamases (SHV, TEM, CTX-M) genes, in Klebsiella pneumoniae isolated from clinical samples in the Ilam city hospitals. In this study, during a one-year period, 100 Klebsiella pneumoniae isolates obtained from patients hospitalized in the Ilam city. All isolates were identified by standard biochemical tests and susceptibility to 11 antibiotics that traditionally use in the treatment of bacterial infection was evaluated using the disk diffusion method. The ESBL producing isolates were identified using the combined disc Test and extended-spectrum betalactamase (TEM, SHV, CTX-M) genes were detected using PCR method. The highest rate of antibiotic resistance among Klebsiella pneumonia isolates was related to ciprofloxacin (100%) and the minimum rate of resistance was related to Imipenem (20 ) (pv≤0.05). Out of 91 isolates were identified as ESBL producing using combined disc method. The PCR results showed that 79 isolates (79%) consisted of CTX-m gene, 91 isolates (91%) were consisting of SHV gene and 77 isolates were containing TEM gene. The high rate of resistance to ESBL enzymes in this study is a greatconcern, so to infection controlling and preventing of spreading drug resistance bacteria highlights the need for infection control measures including antibacterial management andidentification of resistant isolates.

Keywords:

Klebsiella pneumoniae , Ilam , Beta-lactamase , blaSHV , blaTEM , blaCTX-M