Anticoccidial effect of metabolites of native Streptomyces on prevalent eimeria of Iran
Publish Year: 1397
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
BIOCONF20_555
تاریخ نمایه سازی: 28 اردیبهشت 1398
Abstract:
The aim of this study is the isolation and characterization of anticoccidial metabolite producing Streptomyces sp. from Azerbaijan, Iran. Primary screening of the seven strains by TLC and reagent guided to select a novel Streptomyces sp. 6. The antibacterial susceptibility of crude extracts against pathogen microorganisms was assessed by agar disc diffusion method and broth microdilution method. Based on data analysis, HPLC at the 280 nm wavelength at the flow rate of 0.5 ml/min, in the C18 column was used for 2 isolates 6 and 8 which isolate 6 shows peaks as salinomycin antibiotics. Then it was used for OPG test on eimeriosis chickens. It lowered morbidity/mortality rate, decreased oocysts per gram of feces. Collectively, these data demonstrated the potential of isolate 6 to control chicken coccidiosis. Isolate 6 can, therefore, probably be used as an effective means to control coccidiosis. According to the results Improvement of cell culture, media and metabolite production in suspension culture and more can be suggested
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Authors
Alireza Dehnad
Biotechnology Department, East Azerbaijan Research and Education Center Agricultural and Natural Resources, AREEO, Tabriz, Iran,Higher Education Institute of Rab-Rashid, Tabriz, Iran
Maryam Taghavi Narmi
Higher Education Institute of Rab-Rashid, Tabriz, Iran