Genetic diversity of Tanacetum polycephalum populations in West Azerbaijan using ISSR molecular markers

Tanacetum polycephalum from Anthemideae tribe of the Asteraceae family is rangeland species with high morphological diversity and vast dispersal in northwest,east and center of Iran. The purpose of this research is studying genetic diversity of 20 populations of T.polycephalum in West Azerbaijan using ISSR molecular marker. 10 ISSR primers out of 14 primers could be scored based on having or not having a band. Overall 208 bands produced. The range number of bands was between 15(IS24) to 28 (IS20) and an average number of bands was 20.8. Regarding the polymorphism bands percentage, IS8,IS1, IS10, IS13, IS20 markers had a high percentage (100) and IS24 marker had the lower percentage (80) and mean percentage of polymorphism in the primers was %96.03. PIC, RP, EMR and MI means were .30, 6.06, 19.5 and 6.13 respectively. Overall the most effective markers were IS8, IS1, and IS10. The results of the cluster analysis revealed the grouping of populations studied in 2 main and 4 subgroups, in which the different populations collected from the same locality included in one group. Therefore, the close relationship between geographic location and genetic diversity was characterized and it may be concluded that geographic and climatic factors could be one of the responsible main factors for genetic diversity

Keywords:

West Azerbaijan , Cluster analysis , ISSR , Tanacetum polycephalum