Effect of IBA and carbohydrate source on ın vitro rooting from microcuttings of the apple (Malus domestica Borkh cv.Gami Almasi)

Apple is one of the important productions in Iran and all over the world. Gami Almasi is a dwarf rootstock of apple (Malus domestica Borkh) suitable for different cultivars of apple that grow on North West of Iran. The micropropagation is very effective method for propagation in dwarf rootstock of apple. The most likely commercial opportunity for micropropagation of apple is the use of micropropagated plants for conventional propagation either by cuttings or layering. The main purpose of this research were evaluation of two kinds of Carbohydrate Sucrose and Sorbitol (30mg/l) and IBA (0, 1 and 2 mg/l) on the rate of In vitro root regeneration in the leaves in a genotype of Gami Almasi. We used factorial design based on completely randomized design with 4 replications that contain 4 samples in each replication to analyze this exam. The role of IBA and carbohydrate source was significant effect on rooting rate. We found that IBA and carbohydrate source were critical material in the culture medium for root regeneration. The combination of IBA and Sucrose and Sorbitol had the best results in proliferation of apple root. Sucrose (30mg/l) with IBA (2mg/l) showed the higher callus formation and the number of root per cutting

Keywords:

Apple , Carbohydrate , Gami Almasi , In Vitro , Sorbitol