Study of effect of wild type trichoderma fungi and its mutant on germination and some growth traits of cucumber (Cucumis sativus L.) and tomato (Lycopersicon esculentum)

Germination and vegetative components are number of factors that determine final performance of plants. The present study was carried out to determine effect of tomato and cucumber seed biopriming with mixture of five species of Trichoderma antagonist fungus was performed on germination and vegetative traits of seedlings. Also, effect of biopriming with superior mutants of Trichoderma species was one of the goals of this study. Experiments with three treatments: control (non-inoculation), inoculation with bio-formulation prepared from wild type Trichoderma species and inoculation with its mutant isolates, at the Nuclear Agriculture Research Institute in the form of a completely randomized design with three replications was done. Evaluation of traits (germination percentage, stem and root length, fresh and dry weight) were evaluated using standard ISTA method. The results of both experiments showed, Biopriming, significantly increased germination percentage at 1% level. The highest germination percentage of cucumber and tomato seeds, was for Biopriming treatment associated with Trichoderma mutant isolates. However, length of seedlings of cucumber and tomato in the control was higher than treatment with Trichoderma (wild type and mutant). In dry weight index, biopreimine with Trichoderma isolates (parent and mutant) was higher than control its indicate that seedlings were not needed to increase length for producing biomass. In other measured components, there was no statistically significant difference with control. Overall, the results of this study showed, use of seeds biomass with Trichoderma fungi in cucumber and tomato improved most of the plant growth factors, especially during its initial development and growth stages.

Keywords:

Biopriming , Tomato , Cucumber , Germination , Trichoderma